Alternative titles; symbols
HGNC Approved Gene Symbol: UBE2H
Cytogenetic location: 7q32.2 Genomic coordinates (GRCh38) : 7:129,830,732-129,952,960 (from NCBI)
Ubiquitin-conjugating enzymes catalyze the covalent attachment of ubiquitin to cellular substrates. Kaiser et al. (1994) isolated a novel ubiquitin-conjugating enzyme from human placenta and cloned the corresponding cDNA. DNA sequencing revealed that this gene, symbolized UBCH2 by them, encodes a protein with significant sequence similarity to yeast UBC8. They discovered that yeast UBC8 is interrupted by a single intron bearing an unusual branch point sequence. The authors noted that yeast UBC8 exhibited 54% amino acid sequence identity to human UBCH2. Moreover, full-length yeast and human enzymes expressed from the cDNAs showed similar enzymatic activities in vitro by catalyzing the ubiquitination of histones, suggesting that the 2 enzymes may fulfill similar functions in vivo.
By study of hamster/human hybrid cell DNAs, Kaiser et al. (1994) demonstrated that the human UBC8 gene is located on chromosome 7. Hayashida et al. (2000) constructed a 1-Mb physical and transcript map of 7q32 and mapped UBE2H to a region between D7S530 and D7S649.
Hayashida, S., Yamasaki, K., Asada, Y., Soeda, E., Niikawa, N., Kishino, T. Construction of a physical and transcript map flanking the imprinted MEST/PEG1 region at 7q32. Genomics 66: 221-225, 2000. [PubMed: 10860668] [Full Text: https://doi.org/10.1006/geno.2000.6206]
Kaiser, P., Seufert, W., Hofferer, L., Kofler, B., Sachsenmaier, C., Herzog, H., Jentsch, S., Schweiger, M., Schneider, R. Human ubiquitin-conjugating enzyme homologous to yeast UBC8. J. Biol. Chem. 269: 8797-8802, 1994. [PubMed: 8132613]