Alternative titles; symbols
HGNC Approved Gene Symbol: ZNF271P
Cytogenetic location: 18q12 Genomic coordinates (GRCh38) : 18:27,500,001-45,900,000
Zinc finger proteins interact with nucleic acids and have diverse functions. The zinc finger domain is a conserved amino acid sequence motif containing 2 specifically positioned cysteines and 2 histidines that are involved in coordinating zinc. Kruppel-related proteins form 1 family of zinc finger proteins. See ZFP93 (604749) for additional information on zinc finger proteins.
By screening a human monoblast cell line (U-937) cDNA library with a degenerate oligonucleotide based on a conserved region of Kruppel-related zinc finger proteins that encompasses the H/C link, Abrink et al. (1995) isolated cDNAs encoding 42 distinct Kruppel-related zinc finger proteins, including ZNF271, which they called HZF7. The partial ZNF271 cDNA, which lacks 5-prime and 3-prime coding sequence, encodes at least 2 zinc finger motifs. Northern blot analysis detected a major 4.0-kb ZNF271 transcript in HeLa cells, U-937 cells, and PMA-induced U-937 cells.
Epstein-Barr virus (EBV) infects the majority of humans in adolescence, establishes latency in a small subset of B lymphocytes, and transforms cells through the coordinate expression of a selected repertoire of EBV-encoded latency genes. Using cDNA subtraction cloning to identify genes differentially expressed in EBV-infected B cells, Tune et al. (2002) obtained a cDNA encoding ZNFEB. The deduced 423-amino acid protein contains 5 putative zinc-binding, Kruppel-type finger domains in its C-terminal portion, each separated by 7 amino acids. RT-PCR analysis detected expression only in T-cell lines, thymocytes, thyroid, and pancreatic islet cells. Expression in tonsillar B cells was low but increased transiently after B-cell receptor stimulation and mitogenic activation. After EBV infection, however, levels continued to rise, approximating the kinetics of viral latency genes. Treatment of tonsillar B cells with antisense to ZNFEB inhibited EBV-induced growth transformation and ZNFEB expression, similar to the effects of antisense to viral EBNA1 or EBNA2 or cellular LCK (153390). Tune et al. (2002) concluded that ZNFEB is essential in the EBV-mediated transformation process.
By genomic sequence analysis, Tune et al. (2002) determined that the ZNFEB gene contains 1 exon and spans 18 kb.
By genomic sequence analysis, Tune et al. (2002) mapped the ZNFEB gene to chromosome 18q12 between the MAPRE2 (605789) and ZNF24 (194534) genes.
Abrink, M., Aveskogh, M., Hellman, L. Isolation of cDNA clones for 42 different Kruppel-related zinc finger proteins expressed in the human monoblast cell line U-937. DNA Cell Biol. 14: 125-136, 1995. [PubMed: 7865130] [Full Text: https://doi.org/10.1089/dna.1995.14.125]
Tune, C. E., Pilon, M., Saiki, Y., Dosch, H.-M. Sustained expression of the novel EBV-induced zinc finger gene, ZNF-EB, is critical for the transition of B lymphocyte activation to oncogenic growth transformation. J . Immun. 168: 680-688, 2002. [PubMed: 11777961] [Full Text: https://doi.org/10.4049/jimmunol.168.2.680]