Other entities represented in this entry:
HGNC Approved Gene Symbol: ZNF236
Cytogenetic location: 18q23 Genomic coordinates (GRCh38) : 18:76,822,557-76,972,901 (from NCBI)
Zinc finger proteins interact with nucleic acids and have diverse functions. The zinc finger domain is a conserved amino acid sequence motif containing 2 specifically positioned cysteines and 2 histidines that are involved in coordinating zinc (summary by Abrink et al., 1995). ZNF236 is a Kruppel-like zinc finger protein (Holmes et al., 1999).
Using mRNA differential display to identify glucose-regulated genes in human mesangial cells, Holmes et al. (1997) isolated a partial cDNA encoding a novel Kruppel-like zinc finger protein, ZNF236. ZNF236 expression was upregulated by high, pathologic concentrations of D-glucose. By screening a human fetal kidney cDNA library with the partial ZNF236 cDNA, Holmes et al. (1999) isolated cDNAs representing 2 alternatively spliced ZNF236 transcripts, which the authors named ZNF236A and ZNF236B. These transcripts are identical except for the presence of an additional 82-bp exon in ZNF236A that truncates the open reading frame. The ZNF236A cDNA encodes a predicted 1,558-amino acid full-length protein containing 25 C2H2 zinc finger motifs. The ZNF236B cDNA encodes a predicted 1,845-amino acid full-length protein containing 30 C2H2 zinc finger motifs. The zinc fingers are distributed throughout ZNF236A and ZNF236B in clusters of 5 to 9 fingers. Within the clusters, the majority of zinc fingers are connected by a conserved stretch of 7 amino acids, called the H/C link, that is characteristic of the Drosophila repressor protein Kruppel and Kruppel-related zinc finger proteins. An N-terminal Kruppel-associated box (KRAB) is not present in the ZNF236 proteins. Northern blot analysis detected an approximately 8.3-kb ZNF236 transcript in all human tissues tested, with highest expression in skeletal muscle and brain, intermediate expression in heart, pancreas, and placenta, and lowest expression in kidney, liver, and lung. PCR of cDNAs derived from human kidney showed that the ZNF236B transcript is substantially more abundant than the ZNF236A transcript in both adult and fetal kidney. RT-PCR analysis confirmed that the expression of both ZNF236A and ZNF236B is upregulated in human mesangial cells in response to elevated levels of D-glucose.
By bioinformatic and RT-PCR analyses, Halama et al. (2003) identified a third human ZNF236 splice variant, ZNF236C, that encodes a predicted 449-amino acid isoform.
Halama et al. (2003) determined that the ZNF236 gene contains 32 exons.
By analysis of a somatic cell hybrid panel, Holmes et al. (1999) mapped the ZNF236 gene to chromosome 18. Using FISH, they localized the ZNF236 gene to chromosome 18q22-q23.
Gross (2020) mapped the ZNF236 gene to chromosome 18q23 based on an alignment of the ZNF236 sequence (GenBank AF085243) with the genomic sequence.
Abrink, M., Aveskogh, M., Hellman, L. Isolation of cDNA clones for 42 different Kruppel-related zinc finger proteins expressed in the human monoblast cell line U-937. DNA Cell Biol. 14: 125-136, 1995. [PubMed: 7865130] [Full Text: https://doi.org/10.1089/dna.1995.14.125]
Gross, M. B. Personal Communication. Baltimore, Md. 12/11/2020.
Halama, N., Yard-Breedijk, A., Vardarli, I., Akkoyun, I., Yard, B., Janssen, B., van der Woude, F. J. The Kruppel-like zinc-finger gene ZNF236 is alternatively spliced and excluded as susceptibility gene for diabetic nephropathy. Genomics 82: 406-411, 2003. [PubMed: 12906866] [Full Text: https://doi.org/10.1016/s0888-7543(03)00120-4]
Holmes, D. I. R., Wahab, N. A., Mason, R. M. Identification of glucose-regulated genes in human mesangial cells by mRNA differential display. Biochem. Biophys. Res. Commun. 238: 179-184, 1997. [PubMed: 9299475] [Full Text: https://doi.org/10.1006/bbrc.1997.7265]
Holmes, D. I. R., Wahab, N. A., Mason, R. M. Cloning and characterization of ZNF236, a glucose-regulated Kruppel-like zinc-finger gene mapping to human chromosome 18q22-q23. Genomics 60: 105-109, 1999. [PubMed: 10458916] [Full Text: https://doi.org/10.1006/geno.1999.5897]